Agronomic Trait and Genetic Analysis of Latvian Flax Germplasm

Authors

  • Dainis Runģis Genetic Resource Centre, LSFRI „Silava”
  • Linards Ļubinskis Genetic Resource Centre, LSFRI „Silava”
  • Veneranda Stramkale Agriculture Science Centre of Latgale

DOI:

https://doi.org/10.17770/etr2011vol2.1000

Keywords:

flax breeding, DNA markers, characterisation and evaluation

Abstract

There is a long history of flax cultivation in Latvia, and breeding programs were active until 1970’s, when flax breeding in Latvia was halted. Since 1992, the Agriculture Science Centre of Latgale (ASCL) has repatriated Latvian flax germplasm from various genebanks, as well as renewed limited breeding activities in flax. Currently, the ASCL holds a collection of 497 flax accessions, as well as 9865 accessions of various lines and hybrids developed at the LLZC since 1993. To assist in the characterization of this Latvian flax germplasm, we have utilised DNA markers to assess genetic diversity and relatedness, as well as surveying functional polymorphism. We have utilised Simple sequence repeat (SSR) markers developed from both genomic libraries as well as expressed sequences. The results of the DNA marker survey were utilised to determine the genetic polymorphism and relatedness within Latvian flax germplasm, and these results were compared with the analysis of agronomic traits carried out in field trials at the ASCL. The development of DNA markers linked to traits of agronomic importance will assist in the development of a Latvian flax breeding program. The use of DNA marker technology will allow more efficient assessment and rational utilization of Latvian flax germplasm.

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References

Holms, I., Sniedze R. Šķiedraugu selekcija [Fibre crop breeding]. In ‘Laukaugu selekcija Latvijā’ [‘Crop breeding in Latvia’] Holms, I. (ed). Avots, Rīga. (1992) p. 123-130. (in Latvian) 2. Roos-Amsaleg, C., Cariou-Pham, E., Vautrin, D., Tavernier, R., Solignac, M., Molecular Ecology Notes, 2006, v. 6: p.796-799.

Roach, M.J., Deyholos, M.K. Microarray analysis of flax (Linum usitatissimum L.) stems identifies transcripts enriched in fibre-bearing phloem tissues. Molecular Genetic and Genomics, 2007, v. 278 p. 149–165.

Hotte. N.S., Deyholos, M.K. A flax fibre proteome: identification of proteins enriched in bast fibres. BMC Plant Biology, 2008, v. 8:52. 5. Cloutier, S., Niu, Z., Datla, R., Duguid, S. Development and analysis of EST-SSRs for flax (Linum usitatissimum L.) Theoretical and Applied Genetics, 2009, v. 119 p. 53–63.

Doyle, J.J., Doyle, J.L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 1987, v. 19 p. 11-15. 7. Cloutier, S., Ragupathy, R., Niu, Z., Duguid, S. SSR-based linkage map of flax (Linum usitatissimum L.) and mapping of QTLs underlying fatty acid composition traits. Melecular breeding, doi:10.1007/s11032-010-9494-1.

Peakall, R., Smouse P.E. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 2006, v. 6 p. 288-295.

Tamura, K., Dudley, J., Nei, M., Kumar, S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 2007, v. 24 p. 1596-1599.

Rungis, D., Bérubé, Y., Zhuang, J., Ralph, S., Ritland, C.E., Ellis, B.E., Douglas, C., Bohlmann, J., Ritland, K. Robust simple sequence repeat (SSR) markers for spruce (Picea spp.) from expressed sequence tags (ESTs). Theoretical and Applied Genetics, 2004, v. 109 p. 1283-1294.

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Published

2015-08-05

How to Cite

[1]
D. Runģis, L. Ļubinskis, and V. Stramkale, “Agronomic Trait and Genetic Analysis of Latvian Flax Germplasm”, ETR, vol. 2, pp. 260–267, Aug. 2015, doi: 10.17770/etr2011vol2.1000.