APPROBATION OF MICROBIALLY AVAILABLE PHOSPHORUS (MAP) DETERMINATION METHOD BY FLOW CYTOMETRY

Authors

  • Marta Frolova Riga Technical University, Faculty of Civil Engineering, Research Centre for Civil Engineering, Water Research Laboratory (LV)
  • Jurģis Zemītis Riga Technical University, Faculty of Civil Engineering, Research Centre for Civil Engineering, Water Research Laboratory (LV)
  • Kristina Tihomirova Riga Technical University, Faculty of Civil Engineering, Research Centre for Civil Engineering, Water Research Laboratory (LV)
  • Linda Mežule Riga Technical University, Faculty of Civil Engineering, Research Centre for Civil Engineering, Water Research Laboratory (LV)
  • Jānis Rubulis Riga Technical University, Faculty of Civil Engineering, Research Centre for Civil Engineering, Water Research Laboratory (LV)
  • Kamila Gruškeviča Riga Technical University, Faculty of Civil Engineering, Research Centre for Civil Engineering, Water Research Laboratory (LV)
  • Tālis Juhna Riga Technical University, Faculty of Civil Engineering, Research Centre for Civil Engineering, Water Research Laboratory (LV)

DOI:

https://doi.org/10.17770/etr2017vol1.2533

Keywords:

microbially available phosphorus, flow cytometry, drinking water

Abstract

Phosphorus (P) is among the most important nutrients required for bacterial growth. It has a great influence on microbial activity even at very small concentrations. Existing chemical methods are not able to determine P at low enough concentrations and to quantify biologically available phosphorus fractions. Therefore, a method of microbially available phosphorus (MAP) determination is used to quantify the amount of P at concentrations below 20 µg/l. Additionally, this method determines the amount of P that can be directly used by microorganisms. Originally it was determined by inoculating sample by Pseudomonas fluorescens (now Ps. brenneri) P17 strain and spread-plated on R2A agar for enumeration. Further, a more rapid method was developed by replacing heterotrophic plate count (HPC) by flow cytometry (FCM). In this paper the use of FCM for MAP determination is validated and compared with HPC method. The results of calibration are presented. The original pure P17 strain was used as inoculum and standards with different PO4-P concentrations were inoculated at 30°C. The gained yield factor by FCM was 1.59x108. FCM results showed strong correlation (R2=0.99) with HPC results, as pure culture was used. Therefore, flow cytometry is a rapid alternative to heterotrophic plate count method for microbially available phosphorus determination.

Downloads

Download data is not yet available.

References

I. T. Miettinen, T. Vartiainen, and P. J. Martikainen, “Phosphorus and bacterial growth in drinking water,” Appl. Environ. Microbiol., vol. 63, no. 8, pp. 3242–3245, 1997.

M. J. Lehtola, T. Juhna, I. T. Miettinen, T. Vartiainen, and P. J. Martikainen, “Formation of biofilms in drinking water distribution networks, a case study in two cities in Finland and Latvia,” J. Ind. Microbiol. Biotechnol., vol. 31, no. 11, pp. 489–494, 2004.

M. Polanska, K. Huysman, and C. Van Keer, “Investigation of microbially available phosphorus (MAP) in flemish drinking water,” Water Res., vol. 39, no. 11, pp. 2267–2272, 2005.

T. Juhna, “Aspects of Drinking Water Supply in Areas of Humic Water.”

M. J. Lehtola, I. T. Miettinen, T. Vartiainen, and P. J. Martikainen, “A New Sensitive Bioassay for Determination of Microbially Available Phosphorus in Water A New Sensitive Bioassay for Determination of Microbially Available Phosphorus in Water,” vol. 65, no. 5, pp. 5–8, 1999.

G. Wen, Q. Deng, T.-L. Huang, and J. Ma, “An improved method for determining microbially available phosphorus in drinking water,” Water Sci. Technol. Water Supply, 2016.

F. Hammes, M. Berney, Y. Wang, M. Vital, O. Köster, and T. Egli, “Flow-cytometric total bacterial cell counts as a descriptive microbiological parameter for drinking water treatment processes,” Water Res., vol. 42, no. 1–2, pp. 269–277, Jan. 2008.

E. I. Prest, F. Hammes, S. Kötzsch, M. C. M. van Loosdrecht, and J. S. Vrouwenvelder, “Monitoring microbiological changes in drinking water systems using a fast and reproducible flow cytometric method,” Water Res., vol. 47, no. 19, pp. 7131–7142, 2013.

A. Nescerecka, J. Rubulis, M. Vital, T. Juhna, and F. Hammes, “Biological instability in a chlorinated drinking water distribution network,” PLoS One, vol. 9, no. 5, pp. 1–11, 2014.

D. J. Reasoner and E. E. Geldreich, “A new medium for the enumeration and subculture of bacteria from potable water,” Appl. Environ. Microbiol., vol. 49, no. 1, pp. 1–7, Jan. 1985.

D. Jiang, Y. Chen, and G. Ni, “Effects of Total Phosphorus (TP) and Microbially Available Phosphorus (MAP) on Bacterial Regrowth in Drinking Water Distribution System,” Syst. Eng. Procedia, vol. 1, pp. 124–129, 2011.

J. Rubulis and T. Juhna, “Evaluating the potential of biofilm control in water supply systems by removal of phosphorus from drinking water,” Water Sci. Technol., vol. 55, no. 8–9, pp. 211–217, 2007.

[A. Spalviņš, J. Šlangens, I. Lāce, R. Janbickis, I. Eglīte, and T. Juhna, “Baltezera ūdensgūtves režīmu optimizācija un mangāna koncentrācijas dinamikas pētīšana izmantojot nestacionāro hidroģeoloģisko modeli,” Rīga, 2009.

Downloads

Published

2017-06-15

Issue

Vol. 1 (2017): Environment. Technology. Resources. Proceedings of the 11th International Scientific and Practical Conference. Volume 1

Section

Environment and Resources

How to Cite

[1]
M. Frolova, “APPROBATION OF MICROBIALLY AVAILABLE PHOSPHORUS (MAP) DETERMINATION METHOD BY FLOW CYTOMETRY”, ETR, vol. 1, pp. 89–92, Jun. 2017, doi: 10.17770/etr2017vol1.2533.