Impact of Microbiological Fertilizer Baikal EM-1 on Onion Growth in Greenhouse Conditions

Lelde Grantina-Ievina, Lasma Stanke, Guna Ergle


The microbiological fertilizer Baikal EM-1 contains lactic acid bacteria, photosynthetic bacteria, nitrogen fixing bacteria, Saccharomyces yeasts and microbial cultivation media. The aim of the present study was to evaluate the impact of it on onion grown in controlled greenhouse conditions.

In summer 2014 two trials with this product were carried out on onions in greenhouse conditions. Before planting onion bulbs were soaked in water with added fertilizer and growth substrate was watered with the fertilizer according to the instructions of manufacturer. During the vegetation period plants were watered with the fertilizer several times. Identical treatment was performed with water in the control plots. Different treatment schemes and growth substrates (neutralized peat and substrate for vegetables) were used in both trials. The main difference of the used substrates was in the content of mineral nutrients that was higher in the substrate for vegetables. In the second trial additional fertilization with ammonium nitrate was applied three times during the vegetation period. At the end of the trials the yield of onion leaves was estimated, as well as the length of leaves and their chlorophyll content was measured.

At the first trial the obtained yield of onion leaves was increased by 6.4 % and in the second by 8.2 % due to treatment with Baikal EM-1 but these differences were not statistically significant in comparison to the control plots. In general, the fertilizer increased all other measured parameters as well, including average length of leaves by 4.6 % and 1.3 %, the chlorophyll content by 5.0 % and 1.5 %, in the first and second trial respectively, and percentage of onions developing leaves increased by 13.2 % in the second trial. Only the last parameter showed statistically significant differences in comparison to the control. Additionally the growth of the onion was more even in the control treatment. In conclusion, the microbiological fertilizer Baikal EM-1 gave positive impact on onions in greenhouse conditions.


chlorophyll content; microbiological fertilizer; onions; yield

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R. L. Berendsen, C. M. J. Pieterse, P. A. H. M. Bakker, “The rhizosphere microbiome and plant health,” Trends Plant Sci., vol. 17, pp. 478- 486, 2012.

T.-H. Anderson, “Microbial eco-physiological indicators to asses soil quality,” Agric. Ecosyst. Environ., vol. 98, pp. 285-293, 2003.

Y.-S. Chen, F. Yanagida, T. Shinohara, “Isolation and identification of lactic acid bacteria from soil using an enrichment procedure,” Lett. Appl. Microbiol., vol. 40, pp. 195-200, 2005.

M. Kobayashi, M. Z. Haque, “Contribution to nitrogen fixation and soil fertility by photosynthetic bacteria,” Plant Soil, vol. 35, pp. 443-456, 1971.

M. M. Roper, J. K. Ladha, “Biological N2 fixation by heterotrophic and phototrophic bacteria in association with straw,” Dev. Plant Soil Sci., vol. 65, pp. 211-224, 1995.

S. Allahverdiyev, A. Atilla, B. S. Ismail, A. Sahmurova, “Response of photosystem II and photosynthetic pigments to salt and Baikal EM1 in tree seedlings,” Afr. J. Biotechnol., vol. 10, pp. 535-538, 2011.

Z. P. Okazova, M. V. Efakov, “Use of biological products in corn seed farming,” Plant Protect., vol. 40, pp. 261-263, 2014.

A. K. Kulikova, O. S. Dronina, E. A. Nikiforov, “Effect of seed treatment by diatomite powder on soil nutrient status and sugarbeet productivity,” Sakharnaya Svekla, vol. 1, pp. 24-26, 2009.

S. M. Westerveld, A. W. McKeown, M. R. McDonald, “Assessment of chlorophyll and nitrate meters as field tissue nitrogen tests for cabbage, onions, and carrots,” HortTechnology, vol 14, pp. 179-188, Apr.-June. 2004.

I. Tringovska, “The effects of humic and bio-fertilizers on growth and yield of greenhouse tomatoes,” Acta Hort., vol. 960, pp. 443-449, 2012.

A. M. El-Bassiony, “Effect of potassium fertilization on growth, yield and quality of onion plants,” J. Appl. Sci. Res., vol. 2, pp. 780-785, 2006.

A. M. Shaheen, M. M. Abdel-Mouty, A. H. Ali, F. A. Rizk, “Natural and chemical phosphorus fertilizers as affected onion plant growth, bulbs yield and its some physical and chemical properties,” Austr. J. Basic Appl. Sci., vol. 1, pp. 519-524, 2007.

C. Altomare, I. Tringovska, “Beneficial soil microorganisms, an ecological Aalternative for soil fertility management,” Sust. Agric. Rev., vol. 7, pp 161-214, May 2011.



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