EXTRACTION OF BIOLOGICALLY ACTIVE COMPONENTS FROM FRESHWATER SAPROPEL

Aneka Klavina, Agris Auce, Ivars Vanadzins, Alise Silova, Linda Dobkevica

Abstract


Sapropel has been used for different purposes - in agriculture as fertilizer, in construction as building material, in cosmetic products, in balneology also in medicine and pharmaceuticals as bioactive component. Previously sapropel has been commonly used in raw form and there is no general accepted method or standard method for obtaining sapropel extract. However, most extraction methods follow the same path. Currently, there are few extraction methods using several extractants for obtaining bioactive components from raw sapropel. The most commonly used extractant is alkaline solution. When sapropel is subjected to alkaline environment, the humic and fulvic acids, together with some lipids, vitamins and sugar, present in the raw sapropel become soluble, however other organic and mineral content present in the sapropel remain solid. Alkaline extraction is followed by filtration and water present in the aqueous mixture is evaporated off. Latvian freshwater sapropel can be used as raw material for obtaining sapropel extract and use it as remedy. But the main question for sapropel usage in medicine, balneology and pharmacy is to develop quality criteria for raw sapropel and its extracts. The quality criteria should include minimum requirements for biologically active substance concentration, pH values, antioxidants as well as physical characteristics. In future studies the differences in extract characteristics of the various deposit sites, as well as the stability of the extracts under different storage conditions should be defined; also, there is need for a common approach to develop method of extraction process for active substances from sapropel and analysis procedures of its extract.

Keywords


antioxidants; extraction; freshwater sapropel; fulvic acid; humic acid; sapropel

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References


K. Stankeviča and M. Kļaviņš, “Sapropelis un tā izmantošanas iespējas,” Mater. Sci. Appl. Chem., vol. 29, no. 29, p. 109, 2014.

R. Sánchez-Espejo, C. Aguzzi, P. Cerezo, I. Salcedo, A. López-Galindo, and C. Viseras, “Folk pharmaceutical formulations in western Mediterranean: Identification and safety of clays used in pelotherapy,” J. Ethnopharmacol., vol. 155, no. 1, 2014.

M. Orru, M. Übner, and H. Orru, “Chemical properties of peat in three peatlands with balneological potential in Estonia,” no. Wollina 2009, pp. 43–49, 2011.

E. S. Trofimova, M. V Zykova, A. A. Ligacheva, E. Y. Sherstoboev, V. V Zhdanov, M. V Belousov, M. S. Yusubov, S. V Krivoshchekov, M. G. Danilets, and A. M. Dygai, “Influence of Humic Acids Extracted from Peat by Different Methods on Functional Activity of Macrophages in Vitro,” vol. 162, no. 6, pp. 741–742, 2017.

A. Fioravanti, S. Tenti, C. Giannitti, N. A. Fortunati, and M. Galeazzi, “Short- and long-term effects of mud-bath treatment on hand osteoarthritis: A randomized clinical trial,” Int. J. Biometeorol., 2014.

T. Belwal, S. M. Ezzat, L. Rastrelli, I. D. Bhatt, M. Daglia, A. Baldi, H. P. Devkota, I. E. Orhan, J. K. Patra, G. Das, C. Anandharamakrishnan, L. Gomez-Gomez, S. F. Nabavi, S. M. Nabavi, and A. G. Atanasov, “A critical analysis of extraction techniques used for botanicals: Trends, priorities, industrial uses and optimization strategies,” TrAC - Trends Anal. Chem., vol. 100, no. 2018, pp. 82–102, 2018.

G. L. Ryzhova, M. A. Tyunina, and K. A. Dychko, “Determination of fatty acids in products of the vibromagnetic treatment of sapropel by chromatography-mass spectrometry,” J. Anal. Chem., vol. 68, no. 8, pp. 736–742, 2013.

C. C. Xu, B. Wang, Y. Q. Pu, J. S. Tao, and T. Zhang, “Advances in extraction and analysis of phenolic compounds from plant materials,” Chin. J. Nat. Med., vol. 15, no. 10, pp. 721–731, 2017.

J. M. Roux, H. Lamotte, and J. L. Achard, “An Overview of Microalgae Lipid Extraction in a Biorefinery Framework,” Energy Procedia, vol. 112, no. October 2016, pp. 680–688, 2017.

O. Y. Strus, “Study of sapropel extracts from Prybych natural deposits,” J. Chem. Pharm. Res., vol. 7, no. 6, pp. 133–137, 2015.

“Latvian lakes datubase: ezeri.lv,” Datubase. [Online]. Available: https://www.ezeri.lv/database/. [Accessed: 04-Mar-2019].

A. Rebiai, T. Lanez, and M. L. Belfar, “Total polyphenol contents, radical scavenging and cyclic voltammetry of algerian propolis,” Int. J. Pharm. Pharm. Sci., 2014.

M. Tarnawski, K. Depta, D. Grejciun, and B. Szelepin, “HPLC determination of phenolic acids and antioxidant activity in concentrated peat extract - A natural immunomodulator,” J. Pharm. Biomed. Anal., vol. 41, no. 1, pp. 182–188, 2006.

W. Brand-Williams, M. E. Cuvelier, and C. Berset, “Use of a free radical method to evaluate antioxidant activity,” LWT - Food Science and Technology. 1995.

N. J. Miller, C. Rice-Evans, M. J. Davies, V. Gopinathan, and A. Milner, “A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates.,” Clin. Sci., 1993.

G. A. Leonova, V. A. Bobrov, S. K. Krivonogov, A. A. Bogush, V. A. Bychinskii, A. E. Mal’tsev, and G. N. Anoshin, “Biogeochemical specifics of sapropel formation in Cisbaikalian undrained lakes (exemplified by Lake Ochki),” Russ. Geol. Geophys., vol. 56, no. 5, pp. 745–761, May 2015.

A. Javanshah and A. Saidi, “Determination of Humic Acid by Spectrophotometric Analysis in the Soils,” pp. 19–23, 2016.

G. D. S. Tserenpil, O. Ugtakhbayar, S. G. Shevchenko, L. V Kliba, and M. G. Voronkov, “Characterization and organic compounds in peloids from Mongolia,” 2009.

G. P. Alexandrova, G. Dolmaa, B. G. Sukhov, and D. Regdel, “A new humic acid remedy with addition of silver nanoparticles,” vol. 13, pp. 7–11, 2012.

A. Whitbread, “Soil organic matter: its fractionation and role in soil structure,” Soil Org. Matter Manag. Sustain., 1995.




DOI: http://dx.doi.org/10.17770/etr2019vol3.4135

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