• Veslava Matikevičienė JSC “Biocentras” (LT)
  • Danutė Masiliūnienė JSC “Biocentras” (LT)
  • Saulius Grigiškis JSC “Biocentras” (LT)



Feather, feather degrading bacterium, keratins, keratinolytic activity, poultry wastes


The aim of this study was to select keratin-degrading bacteria from JSC “Biocentras” collection and poultry processing plant wastewater, and to study their ability to degrade chicken feathers. Isolated from poultry processing plant wastewater bacteria was grown in basal media with feathers meal and showed high keratinolytic activity and protein content throughout the cultivation time. Bacterial strains B. licheniformis 511, B. subtilis I1, B. subtilis 717, and B. subtilis 103 suggested strongly of bacteria that produces keratinolytic activity in the cell free culture supernatants. The obtained results showed that maximum activity of keratinase is a function of cultivation time by the bacteria tested. B. subtilis 103 reached to its maximum level of keratinase production (152 U/mL) after 24 hrs, when over bacteria (148-242 U/mL) after 48 hrs. The good ability of selected bacteria to degrade feathers was detected. The best biodegradation of feathers was obtained using B. subtilis I-1. Over bacillus good degraded feathers as well.


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Kim J.D. Purification and Characterization of a Keratinase from a Feather-Degrading Fungus, Aspergillus flavus Strain K-03. Microbiology, No.35(4), 2007. p. 219-225.

Esawy M.A. Isolation and Partial Characterization of Extracellular Keratinase from a Novel Mesophilic Streptomyces albus AZA. Research Journal of Agriculture and Biological Sciences, No.3(6), 2007. p. 808-817.

Krelpak L., Doucet J., Briki F. New aspects of the α-helix to β-sheets transition in stretched hard α-keratin fibers. Biophysics Journal, No.87, 2004. p. 640-647.

Gupta R., Ramnani P. Microbial keratinases and their prospective applications: an overview. Applied Microbiology and Biotechnology, No.70, 2006. p. 21-33.

Korkmaz H., Hür H., Dinçer S. Characterization of alkaline keratinase of Bacillus licheniformis strain HK-1 from poultry waste. Annals of Microbiology, No.54(2), 2004. p. 201-211.

Hoq M.M., Siddiquee K.A.Z., Kawasaki H., Seki T. Keratinolytic activity of some newly isolated Bacillus species. Journal of Biological Sciences, No.5(2), 2005. p. 193-200.

Gradišar H., Friedrich J., Križaj I., Jerala R. Similarities and Specifities of Fungal Keartinolytic Proteases: Comparison of Keratinases of Paecilomyces marquandii and Doratomyces microsporus to some Known Proteases. Applied and Enviromental Microbiology, No.71(7), 2005. p. 3420-3426.

Tapia D.M.T., Contiero J. Production and partial characterization of keratinase produced by a microorganism isolated from poultry processing plant wastewater. African Journal of Biotechnology, No.7(3), 2008. p. 296-300.

Gousterova A., Braikova D., Haertle T., Nedkov P. Degradation of keratin and collagen containing wastes by newly isolated thermoactinomycetes or by alkaline hydrolysis. Letters in Applied Microbiology, No.40, 2005. p. 335-340.

Brutt E.H.; Ichida J.M. 2001.04.10. Bacteria useful for degrading Keratin. United States Patent No. 6, 214, 576 B1. 7 p.

Joshi S.G., Tejashwini M.M., Revati N.; Sridevi R., Roma D. Isolation, identification and characterazation of a feather degrading bacterium. International Journal of Poultry Science, No.6(9), 2007,. p. 689-693.

Cai C., Lou B., Zheng X. Keratinase production and keratin degradation by a mutant strain of Bacillus subtilis. Journal of Zhejiang University Science B, No.9(1), 2008. p. 60-67.

Cortezi M., Contiero J., Lima C.J.B., Lovaglio R.B., Monti R. Characterization of a feather degrading by Bacillus amyloliquefaciens protease: A new strain. World Journal of Agricultural Sciences, No.4(5), 2008. p. 648-656.

Sangali S., Brandelli A. Isolation and characterization of a novel feather-degrading bacterial strain. Applied Biochemistry and Biotechnology, No.87, 2000. p. 17-24.

Lee H., Suh D.B., Hwang,J.H., Suh H.J. Characterization of a keratinolytic metalloprotease from Bacillus sp. SCB-3. applied Biochemistry and Biotechnology, No.97, 2002. p. 123-133.

Cao Z.-J., Zhang Q., Wei D.-K., Chen L., Wang J., Zhang X.-Q., Zhou M.-H. Characterization of a novel Stenotrophomonas isolate with high keratinase activity and purification of the enzyme. Journal of Industrial Microbiology and Biotechnology, No.36, 2009. p. 181-188.

Macedo A.J., Beys da Silva W.O., Gava R., Driemeier D., Henriques J.A., Termignoni C. Novel keratinase from Bacillus subtilis S14 exhibiting remarkable dehairing capabilities. Applied and Environmantal Microbiology, No.71(1), 2005. p. 594-596.

Friedrich J., Gradišar H., Mandin D., Chaumont J.P. Screening fungi for synthesis of keratinolytic enzymes. Letters in Applied Microbiology, No.28, 1999. p. 127-130.

Soomoro I.H., Kazi Y.F., Zardari M., Shar A.H. Isolation of keratinophilic fungi from soil in Khairpur city, Sindh, Pakistan. Bangladesh Journal of Microbiology, No.24(1), 2007. p. 79-80.

Lowry O.H., Roserbrough N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent. The Journal of Biological Chemistry, No.193, 1951. p. 265-275.




How to Cite

V. Matikevičienė, D. Masiliūnienė, and S. Grigiškis, “DEGRADATION OF KERATIN CONTAINING WASTES BY BACTERIA WITH KERATINOLYTIC ACTIVITY”, ETR, vol. 1, pp. 284–289, Aug. 2015, doi: 10.17770/etr2009vol1.1107.