Determination and Separation of Diarylheptanoids From Alder Growing in Latvia


  • Liga Roze Latvian State Institute of Wood Chemistry, University of Latvia (LV)
  • Oskars Bikovens Latvian State Institute of Wood Chemistry
  • Galina Telysheva Latvian State Institute of Wood Chemistry



alnus species, bark extracts, diarylheptanoids, oregonin, 1, 7-bis-(3, 4-dihydroxyphenyl)-3- hydroxyheptane-5-O-β-D-xylop


The composition of diarylhepatnoids fraction isolated from bark of two alder species (grey alder and black alder) was studied. The efficiency of three extraction methods used for isolation of diarylhepatnoids from alder bark was compared. Two diarylhepatnoids: 1,7-bis-(3,4-dihydroxyphenyl)-heptan- 3-one-5-O-β-D-xylopyronoside (oregonin) and 1,7-bis-(3,4-dihydroxyphenyl)-3-hydroxyheptane-5-O-β-D- xylopyranoside were isolated from the bark of grey alder. The phenolic components of the extracts were analyzed by high-performance liquid chromatography (HPLC). Quantitative determination of oregonin was performed using an internal standard method. The results obtained show that alder barks are rich source of diarylhepatnoids.


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Pietarinen S., Willför S., Ahotupa M., Hemming J. and Holmbom B. Knotwood and bark extracts: strong antioxidants from waste materials. J. Wood Sci., 52, 2006, pp. 436-444.

Pavlovics G., Antons A., Alksne A., Lavnikovica I., Cirule D., Dolacis J., Daugavietis M., Daugaviete M.Comparison of the anatomical structure elements and physical properties of the wood of different alder species growing in Latvia. Annals of Warsaw University of Life Sciences - SGGW, Forestry and Wood Technology, 69, 2009, pp. 173-177.

Kuroyanagi M., Shimomae M., Nagashima Y., Muto N., Okuda T. New diarylhepatnoids from Alnus japonica nad Their Antioxidative Activity. Chem. Pharm. Bull. 53, 2005, pp. 1519-1523.

Kim H.J., Kim K.H., Yeom S.H., Kim M.K., Shim J.G., Lim H. W., Lee M.W. New diarylheptanoid from the barks of Alnus japonica Steudel. Chin. Chem. Lett., 16, 2005, pp. 1337-1340.

Guz N.R., Lorenz P. and Métraux J.-P. Oregonin from the bark of European Alnus species. Biochem. Syst. Ecol. 30, 2002, pp. 471–474.

Kuo C.H., Lee C.W., Lai Y.C. and Lee S.S. Determination of oregonin in Alnus plants and biological samples by capillary electrophoresis. J. Pharm. Biomed. Anal. 47, 2008, pp. 195-200.

Shen J., Shao X. A comparison of accelerated solvent extraction, Soxhlet extraction, and ultrasonic-assisted extraction for analysis of terpenoids and sterols in tobacco. Anal Bioanal Chem, 383, 2005, pp. 1003–1008.

Suga T., Ohta S.,Hirata T., Aoki T. The absolute confuguration of diarylheptanoid xyloside, oregonin, isolated from the female flowers of Alnus serrulatoides. Chem. Lett., 1982, pp. 895-898.




How to Cite

L. Roze, O. Bikovens, and G. Telysheva, “Determination and Separation of Diarylheptanoids From Alder Growing in Latvia”, ETR, vol. 1, pp. 329–332, Aug. 2015, doi: 10.17770/etr2011vol1.916.